PrimePCR™ Probe Assay: TRAF2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TRAF2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TRAF2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027765
Assay Design:   Intron-spanning
Chromosome Location:   9:139793249-139794083question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000419057 ENST00000429509 ENST00000247668 ENST00000371645 ENST00000414589 ENST00000432785 ENST00000536468 ENST00000359662

Gene Information

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs) and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD a TNF receptor associated apoptotic signal transducer ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1 an apoptosis inhibitor possessing ubiquitin ligase activity can unbiquitinate and induce the degradation of this protein and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene but the biological validity of only one transcript has been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   TRAF2
Gene Name:   TNF receptor-associated factor 2
Aliases:   MGC:45012, TRAP, TRAP3
RefSeq:   NC_000009.11 NT_024000.16
Ensembl:   ENSG00000127191
Entrez:   7186
Chromosome Mapping:   9q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.170000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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