PrimePCR™ Probe Assay: COPS7B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027712
Assay Design:   Intron-spanning
Chromosome Location:   2:232660932-232663656question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000410024 ENST00000409295 ENST00000409091 ENST00000350033 ENST00000412591 ENST00000410017 ENST00000373608 ENST00000449174 ENST00000537799

Gene Information

Description Not Available

Gene Symbol:   COPS7B
Gene Name:   COP9 constitutive photomorphogenic homolog subunit 7B (Arabidopsis)
Aliases:   CSN7B, FLJ12612, MGC111077
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000144524
Entrez:   64708
UniGene:   Hs.335061
Chromosome Mapping:   2q37.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.290000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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