PrimePCR™ Probe Assay: PTPRK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027651
Assay Design:   Intron-spanning
Chromosome Location:   6:128505758-128540131question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000368226 ENST00000532331 ENST00000368213 ENST00000368210 ENST00000368215 ENST00000368207 ENST00000490332 ENST00000368227 ENST00000427676

Gene Information

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. This PTP possesses an extracellular region a single transmembrane region and two tandem catalytic domains and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain an Ig-like domain and four fibronectin type III-like repeats. This PTP was shown to mediate homophilic intercellular interaction possibly through the interaction with beta- and gamma-catenin at adherens junctions. Expression of this gene was found to be stimulated by TGF-beta 1 which may be important for the inhibition of keratinocyte proliferation. [provided by RefSeq Jul 2008]

Gene Symbol:   PTPRK
Gene Name:   protein tyrosine phosphatase, receptor type, K
Aliases:   DKFZp686C2268, DKFZp779N1045, R-PTP-kappa
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000152894
Entrez:   5796
UniGene:   Hs.155919
Chromosome Mapping:   6q22.2-q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 35.440000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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