PrimePCR™ Probe Assay: WDFY3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: WDFY3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: WDFY3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027650
Assay Design:   Intron-spanning
Chromosome Location:   4:85614084-85617160question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000295888 ENST00000514711 ENST00000322366

Gene Information

This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However it is not necessary for starvation-induced macroautophagy. [provided by RefSeq May 2010]

Gene Symbol:   WDFY3
Gene Name:   WD repeat and FYVE domain containing 3
Aliases:   ALFY, KIAA0993, MGC16461, ZFYVE25
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000163625
Entrez:   23001
Chromosome Mapping:   4q21.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.270000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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