PrimePCR™ Probe Assay: SIGLEC10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SIGLEC10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SIGLEC10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027627
Assay Design:   Intron-spanning
Chromosome Location:   19:51917019-51917764question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000432469 ENST00000442846 ENST00000356298 ENST00000339313 ENST00000439889 ENST00000436984 ENST00000525998 ENST00000441969 ENST00000353836

Gene Information

SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs or ITIMs. SIGLECs are typically expressed on cells of the innate immune system with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM Jul 2002]

Gene Symbol:   SIGLEC10
Gene Name:   sialic acid binding Ig-like lectin 10
Aliases:   MGC126774, PRO940, SIGLEC-10, SLG2
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000142512
Entrez:   89790
Chromosome Mapping:   19q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.510000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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