PrimePCR™ Probe Assay: TANK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TANK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TANK, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027612
Assay Design:   Intron-spanning
Chromosome Location:   2:162036172-162060054question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000457476 ENST00000456358 ENST00000403609 ENST00000405852 ENST00000429217 ENST00000406287 ENST00000402568 ENST00000432002 ENST00000392749 ENST00000440506 ENST00000259075

Gene Information

The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1 TRAF2 or TRAF3 thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example the protein encoded by this gene can block TRAF2 binding to LMP1 the Epstein-Barr virus transforming protein and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Nov 2010]

Gene Symbol:   TANK
Gene Name:   TRAF family member-associated NFKB activator
Aliases:   I-TRAF, TRAF2
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000136560
Entrez:   10010
Chromosome Mapping:   2q24-q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.830000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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