PrimePCR™ Probe Assay: PPP6R2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PPP6R2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPP6R2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027508
Assay Design:   Intron-spanning
Chromosome Location:   22:50845202-50853048question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000359139 ENST00000395741 ENST00000395744 ENST00000216061

Gene Information

Protein phosphatase regulatory subunits such as SAPS2 modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity recruiting substrates and determining the intracellular localization of the holoenzyme. SAPS2 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C; MIM 612725) (Stefansson and Brautigan 2006 [PubMed 16769727]).[supplied by OMIM Nov 2010]

Gene Symbol:   PPP6R2
Gene Name:   protein phosphatase 6, regulatory subunit 2
Aliases:   KIAA0685, PP6R2, SAP190, SAPS2, dJ579N16.1
RefSeq:   NC_000022.10 NT_011526.7
Ensembl:   ENSG00000100239
Entrez:   9701
Chromosome Mapping:   22q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.990000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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