PrimePCR™ Probe Assay: NTN4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NTN4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NTN4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027231
Assay Design:   Intron-spanning
Chromosome Location:   12:96077418-96104313question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000343702 ENST00000344911 ENST00000538383 ENST00000553059

Gene Information

NTN4 belongs to a family of proteins related to laminins (see LAMA1 MIM 150320) Koch et al. (2000) [PubMed 11038171].[supplied by OMIM Mar 2008]

Gene Symbol:   NTN4
Gene Name:   netrin 4
Aliases:   FLJ23180, PRO3091
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000074527
Entrez:   59277
Chromosome Mapping:   12q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.170000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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