PrimePCR™ Probe Assay: HNRPLL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HNRPLL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HNRPLL, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027172
Assay Design:   Intron-spanning
Chromosome Location:   2:38812973-38818764question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000449105 ENST00000409636 ENST00000409328 ENST00000410076 ENST00000425682 ENST00000378915 ENST00000358367

Gene Information

HNRNPLL is a master regulator of activation-induced alternative splicing in T cells. In particular it alters splicing of CD45 (PTPRC; MIM 151460) a tyrosine phosphatase essential for T-cell development and activation (Oberdoerffer et al. 2008 [PubMed 18669861]).[supplied by OMIM Aug 2008]

Gene Symbol:   HNRPLL
Gene Name:   heterogeneous nuclear ribonucleoprotein L-like
Aliases:   SRRF, hnRNPLL
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000143889
Entrez:   92906
Chromosome Mapping:   2p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.390000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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