PrimePCR™ Probe Assay: PSEN2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027160
Assay Design:   Intron-spanning
Chromosome Location:   1:227073358-227076584question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000366783 ENST00000422240 ENST00000366782 ENST00000472139 ENST00000391872 ENST00000340188

Gene Information

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase an enzyme that cleaves APP. Also it is thought that the presenilins are involved in the cleavage of the Notch receptor such that they either directly regulate gamma-secretase activity or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   PSEN2
Gene Name:   presenilin 2 (Alzheimer disease 4)
Aliases:   AD3L, AD4, CMD1V, PS2, STM2
RefSeq:   NC_000001.10 NG_007381.1 NT_167186.1
Ensembl:   ENSG00000143801
Entrez:   5664
UniGene:   Hs.25363
Chromosome Mapping:   1q31-q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 34.680000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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