PrimePCR™ Probe Assay: SPTB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SPTB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPTB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027124
Assay Design:   Intron-spanning
Chromosome Location:   14:65251061-65252494question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000556626 ENST00000389721 ENST00000389723 ENST00000389722 ENST00000335612 ENST00000542895 ENST00000389720

Gene Information

This locus encodes a member of the spectrin gene family. Spectrin proteins along with ankyrin play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes and mutations in this gene have been associated with spherocytosis type 2 hereditary elliptocytosis and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq Nov 2009]

Gene Symbol:   SPTB
Gene Name:   spectrin, beta, erythrocytic
Aliases:   EL3, HS2, HSPTB1, SPH2
RefSeq:   NC_000014.8 NT_026437.12 NG_016202.1
Ensembl:   ENSG00000070182
Entrez:   6710
Chromosome Mapping:   14q23-q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.780000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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