PrimePCR™ Probe Assay: CTNNBL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CTNNBL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CTNNBL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027120
Assay Design:   Intron-spanning
Chromosome Location:   20:36406087-36407715question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000361383 ENST00000373473 ENST00000373469 ENST00000405275

Gene Information

The protein encoded by this gene contains an acidic domain a putative bipartite nuclear localization signal a nuclear export signal a leucine-isoleucine zipper and phosphorylation motifs. In addition the encoded protein contains Armadillo/beta-catenin-like repeats which have been implicated in protein-protein interactions. Although the function of this protein has not been determined the C-terminal portion of the protein has been shown to possess apoptosis-inducing activity. [provided by RefSeq Jul 2008]

Gene Symbol:   CTNNBL1
Gene Name:   catenin, beta like 1
Aliases:   C20orf33, FLJ21108, NAP, NYD-SP19, P14L, PP8304, dJ633O20.1
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000132792
Entrez:   56259
Chromosome Mapping:   20q11.23-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.400000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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