PrimePCR™ Probe Assay: SEC16A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SEC16A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SEC16A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027095
Assay Design:   Intron-spanning
Chromosome Location:   9:139355688-139357410question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000277537 ENST00000453963 ENST00000371706 ENST00000313050 ENST00000290037 ENST00000431893 ENST00000404925

Gene Information

The selective export of proteins and lipids from the endoplasmic reticulum (ER) is mediated by the coat protein complex II (COPII) that assembles at discrete sites on the membrane known as endoplasmic reticulum exit sites (ERES) or transitional ER sites. SEC16A is a peripheral membrane protein that defines ERES in mammalian cells and is required for protein transport from ER to Golgi (Watson et al. 2006 [PubMed 17005010]).[supplied by OMIM Jun 2009]

Gene Symbol:   SEC16A
Gene Name:   SEC16 homolog A (S. cerevisiae)
Aliases:   FLJ26737, KIAA0310, RP11-413M3.10, SEC16L, p250
RefSeq:   NC_000009.11 NG_016126.1 NT_024000.16
Ensembl:   ENSG00000148396
Entrez:   9919
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.870000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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