PrimePCR™ Probe Assay: SLC7A10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026661
Assay Design:   Intron-spanning
Chromosome Location:   19:33703812-33706722question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000253188

Gene Information

SLC7A10 in association with 4F2HC (SLC3A2; MIM 158070) mediates high-affinity transport of D-serine and several other neutral amino acids (Nakauchi et al. 2000 [PubMed 10863037]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC7A10
Gene Name:   solute carrier family 7, (neutral amino acid transporter, y+ system) member 10
Aliases:   FLJ20839, HASC-1, asc-1
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000130876
Entrez:   56301
UniGene:   Hs.58679
Chromosome Mapping:   19q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.410000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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