PrimePCR™ Probe Assay: GALNS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GALNS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GALNS, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026557
Assay Design:   Intron-spanning
Chromosome Location:   16:88904106-88907443question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000268695 ENST00000542788 ENST00000439266

Gene Information

This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans keratan sulfate and chondroitin 6-sulfate. Sequence alterations including point missense and nonsense mutations as well as those that affect splicing result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome a lysosomal storage disorder. [provided by RefSeq Jul 2008]

Gene Symbol:   GALNS
Gene Name:   galactosamine (N-acetyl)-6-sulfate sulfatase
Aliases:   FLJ17434, FLJ42844, FLJ98217, GALNAC6S, GAS, MPS4A
RefSeq:   NC_000016.9 NG_008013.1 NG_008667.1 NT_010542.15
Ensembl:   ENSG00000141012
Entrez:   2588
Chromosome Mapping:   16q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.780000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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