PrimePCR™ Probe Assay: LEPREL4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LEPREL4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LEPREL4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026478
Assay Design:   Intron-spanning
Chromosome Location:   17:39959566-39963091question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000355468 ENST00000393928 ENST00000545545

Gene Information

This nucleolar protein was first characterized because it was an autoantigen in cases on interstitial cystitis. The protein with a predicted molecular weight of 50 kDa appears to be localized in the particulate compartment of the interphase nucleolus with a distribution distinct from that of nucleolar protein B23. During mitosis it is associated with chromosomes. [provided by RefSeq Jul 2008]

Gene Symbol:   LEPREL4
Gene Name:   leprecan-like 4
Aliases:   NO55, NOL55, SC65
RefSeq:   NC_000017.10 NG_015860.1 NT_010783.15
Ensembl:   ENSG00000141696
Entrez:   10609
Chromosome Mapping:   17q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 35.620000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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