PrimePCR™ Probe Assay: MTMR7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MTMR7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MTMR7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026442
Assay Design:   Intron-spanning
Chromosome Location:   8:17163383-17169048question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000180173 ENST00000521857 ENST00000398099

Gene Information

This gene encodes a member of the myotubularin family of tyrosine/dual-specificity phosphatases. The encoded protein is characterized by four distinct domains that are conserved among all members of the myotubularin family: the glucosyltransferase Rab-like GTPase activator and myotubularins domain the Rac-induced recruitment domain the protein tyrosine phosphatases and dual-specificity phosphatases domain and the suppressor of variegation 3-9 enhancer-of-zeste and trithorax interaction domain. This protein dephosphorylates the target substrates phosphatidylinositol 3-phosphate and inositol 13-bisphosphate. A pseudogene of this gene is found on chromosome 5. [provided by RefSeq Mar 2009]

Gene Symbol:   MTMR7
Gene Name:   myotubularin related protein 7
Aliases:   DKFZp781E194, MGC163449, MGC163451
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000003987
Entrez:   9108
Chromosome Mapping:   8p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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