PrimePCR™ Probe Assay: CD9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CD9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CD9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026360
Assay Design:   Intron-spanning
Chromosome Location:   12:6342585-6344462question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000382518 ENST00000536586 ENST00000382519 ENST00000009180 ENST00000382515 ENST00000425469 ENST00000543424

Gene Information

This gene encodes a member of the transmembrane 4 superfamily also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation adhesion and signal transduction and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq Jan 2011]

Gene Symbol:   CD9
Gene Name:   CD9 molecule
Aliases:   BTCC-1, DRAP-27, FLJ99568, MIC3, MRP-1, TSPAN-29, TSPAN29
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000010278
Entrez:   928
Chromosome Mapping:   12p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.890000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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