PrimePCR™ Probe Assay: RCBTB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RCBTB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RCBTB1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026354
Assay Design:   Intron-spanning
Chromosome Location:   13:50134163-50140799question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000258646 ENST00000546015 ENST00000378302

Gene Information

This gene encodes a protein with an N-terminal RCC1 domain and a C-terminal BTB (broad complex tramtrack and bric-a-brac) domain. In rat over-expression of this gene in vascular smooth muscle cells induced cellular hypertrophy. In rat the C-terminus of RCBTB1 interacts with the angiotensin II receptor-1A. In humans this gene maps to a region of chromosome 13q that is frequently deleted in B-cell chronic lymphocytic leukemia and other lymphoid malignancies. [provided by RefSeq Jul 2008]

Gene Symbol:   RCBTB1
Gene Name:   regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 1
Aliases:   CLLD7, CLLL7, GLP, MGC33184, RP11-185C18.1
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000136144
Entrez:   55213
Chromosome Mapping:   13q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.740000
Efficiency 107

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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