PrimePCR™ Probe Assay: ANXA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026090
Assay Design:   Intron-spanning
Chromosome Location:   9:75775719-75777771question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000257497 ENST00000456643 ENST00000376911

Gene Information

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35000 to 40000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation prostaglandins and leukotrienes annexin I may have potential anti-inflammatory activity. [provided by RefSeq Jul 2008]

Gene Symbol:   ANXA1
Gene Name:   annexin A1
Aliases:   ANX1, LPC1
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000135046
Entrez:   301
UniGene:   Hs.494173
Chromosome Mapping:   9q12-q21.2,9q21.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.430000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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