PrimePCR™ Probe Assay: IL33, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL33, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL33, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026084
Assay Design:   Intron-spanning
Chromosome Location:   9:6254517-6256061question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000381434 ENST00000417746 ENST00000456383

Gene Information

IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g. IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203) an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al. 2010 [PubMed 20926795]).[supplied by OMIM Jan 2011]

Gene Symbol:   IL33
Gene Name:   interleukin 33
Aliases:   C9orf26, DKFZp586H0523, DVS27, NF-HEV, NFEHEV, RP11-575C20.2
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000137033
Entrez:   90865
Chromosome Mapping:   9p24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.710000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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