PrimePCR™ Probe Assay: IL16, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL16, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL16, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0025976
Assay Design:   Intron-spanning
Chromosome Location:   15:81593750-81595953question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000394660 ENST00000302987 ENST00000394652 ENST00000360547 ENST00000355368 ENST00000329842 ENST00000394653 ENST00000394656

Gene Information

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant a modulator of T cell activation and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq Feb 2010]

Gene Symbol:   IL16
Gene Name:   interleukin 16 (lymphocyte chemoattractant factor)
Aliases:   FLJ16806, FLJ42735, FLJ44234, LCF, NIL16, PRIL16, prIL-16
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000172349
Entrez:   3603
Chromosome Mapping:   15q26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.870000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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