PrimePCR™ SYBR® Green Assay: LDB3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best negative control; Same primer pair as used in probe assay qHsaCIP0058534

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LDB3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LDB3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0057429
Assay Design:   Intron-spanning
Chromosome Location:   10:88428440-88439229question
Amplicon Length:   178
Splice Variants Targeted:   ENST00000372066 ENST00000361373 ENST00000429277 ENST00000458213 ENST00000352360 ENST00000263066 ENST00000372056 ENST00000310944 ENST00000542786

Gene Information

This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1 2 and 5) have C-terminal LIM domains. [provided by RefSeq Jan 2010]

Gene Symbol:   LDB3
Gene Name:   LIM domain binding 3
Aliases:   CMD1C, CYPHER, FLJ35865, KIAA01613, KIAA0613, LDB3Z1, LDB3Z4, LVNC3, ORACLE, PDLIM6, ZASP
RefSeq:   NC_000010.10 NT_030059.13 NG_008876.1
Ensembl:   ENSG00000122367
Entrez:   11155
Chromosome Mapping:   10q22.3-q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.320000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download