PrimePCR™ SYBR® Green Assay: DOK3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCIP0057887

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DOK3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DOK3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0056782
Assay Design:   Intron-spanning
Chromosome Location:   5:176935450-176936522question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000311208 ENST00000340951 ENST00000342129 ENST00000400358 ENST00000400359 ENST00000356166 ENST00000568722 ENST00000421030 ENST00000409996 ENST00000395690 ENST00000339553 ENST00000545244 ENST00000454855 ENST00000229268 ENST00000389231 ENST00000312943 ENST00000357198 ENST00000501403 ENST00000510380 ENST00000506493 ENST00000502885 ENST00000510898 ENST00000372105 ENST00000413052 ENST00000369645 ENST00000369644 ENST00000357443 ENST00000303407 ENST00000590335 ENST00000248139 ENST00000535977 ENST

Gene Information

Description Not Available

Gene Symbol:   DOK3
Gene Name:   docking protein 3
Aliases:   DOKL, FLJ22570, FLJ39939
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000146094
Entrez:   79930
Chromosome Mapping:   5q35.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.910000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download