PrimePCR™ SYBR® Green Assay: FAM75A5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041851

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FAM75A5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FAM75A5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0041782
Assay Design:   Intron-spanning
Chromosome Location:   9:41500848-41503095question
Amplicon Length:   197
Splice Variants Targeted:   ENST00000573833 ENST00000342216 ENST00000242783 ENST00000373779 ENST00000428026 ENST00000460170 ENST00000356870 ENST00000323874 ENST00000293825 ENST00000426706 ENST00000263686 ENST00000367786 ENST00000367788 ENST00000367793 ENST00000367794 ENST00000367791 ENST00000333651 ENST00000377647 ENST00000456183 ENST00000356699 ENST00000377509 ENST00000429767 ENST00000377621 ENST00000332857 ENST00000355045 ENST00000373095 ENST00000373084 ENST00000540353 ENST00000542092 ENST00000349767 ENST00000393592 ENST

Gene Information

Description Not Available

Gene Symbol:   FAM75A5
Gene Name:   family with sequence similarity 75, member A5
Aliases:   Not Available
RefSeq:   NC_000009.11 NT_078043.5
Ensembl:   ENSG00000233788
Entrez:   727905
Chromosome Mapping:   9p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.070000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download