PrimePCR™ SYBR® Green Assay: FAM75A7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041838

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FAM75A7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FAM75A7, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0041769
Assay Design:   Intron-spanning
Chromosome Location:   9:65507507-65509421question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000377647 ENST00000456183 ENST00000356699 ENST00000377509 ENST00000429767 ENST00000377621 ENST00000332857 ENST00000355045 ENST00000014914 ENST00000515820 ENST00000262940 ENST00000298552 ENST00000440111 ENST00000545250

Gene Information

Description Not Available

Gene Symbol:   FAM75A7
Gene Name:   family with sequence similarity 75, member A7
Aliases:   AEP1, C9orf36A
RefSeq:   NC_000009.11 NT_078052.4
Ensembl:   ENSG00000234734
Entrez:   26165
Chromosome Mapping:   9q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.060000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download