PrimePCR™ SYBR® Green Assay: GAGE2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041804

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GAGE2B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GAGE2B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0041735
Assay Design:   Intron-spanning
Chromosome Location:   X:49235717-49236872question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000373563 ENST00000409480 ENST00000409547 ENST00000409196 ENST00000409451 ENST00000373566 ENST00000442437 ENST00000381751 ENST00000402590 ENST00000404720 ENST00000415752 ENST00000381708 ENST00000381725 ENST00000420398 ENST00000405679 ENST00000381698 ENST00000440137 ENST00000445148 ENST00000381722 ENST00000362097 ENST00000381709 ENST00000162330 ENST00000393422 ENST00000535626 ENST00000538440 ENST00000418647 ENST00000542031 ENST00000420641 ENST00000393420 ENST00000546196 ENST00000369360 ENST

Gene Information

Description Not Available

Gene Symbol:   GAGE2B
Gene Name:   G antigen 2B
Aliases:   GAGE-2B, GAGE-2C, GAGE2A, GAGE2C
RefSeq:   NC_000023.10 NT_079573.4
Ensembl:   ENSG00000231850
Entrez:   645037
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.100000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download