PrimePCR™ SYBR® Green Assay: POM121, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041692

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: POM121, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: POM121, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038970
Assay Design:   Intron-spanning
Chromosome Location:   7:72411467-72412419question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000409719 ENST00000567020 ENST00000293970 ENST00000564879 ENST00000299824 ENST00000438192 ENST00000373331 ENST00000305799 ENST00000367342 ENST00000413687 ENST00000367267 ENST00000367268 ENST00000395270 ENST00000358357 ENST00000434423 ENST00000446813 ENST00000257622 ENST00000257665 ENST00000453279 ENST00000307142 ENST00000429713 ENST00000434046 ENST00000379558 ENST00000403861 ENST00000329255 ENST00000220062 ENST00000421977 ENST00000409105 ENST00000409913 ENST00000319466 ENST00000409800 ENST

Gene Information

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits many of which belong to the nucleoporin family. This gene encodes a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene and multiple pseudogenes have been identified. [provided by RefSeq Mar 2012]

Gene Symbol:   POM121
Gene Name:   POM121 membrane glycoprotein
Aliases:   DKFZp586G1822, DKFZp586P2220, FLJ41820, KIAA0618, MGC3792, POM121A
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000196313
Entrez:   9883
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.820000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download