PrimePCR™ SYBR® Green Assay: HIRA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041635

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HIRA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HIRA, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038913
Assay Design:   Intron-spanning
Chromosome Location:   22:19363275-19365475question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000371276 ENST00000371274 ENST00000340170 ENST00000263208 ENST00000541063 ENST00000546308 ENST00000423400 ENST00000437048 ENST00000291074 ENST00000571805 ENST00000446250 ENST00000593536 ENST00000599450 ENST00000595426 ENST00000594867

Gene Information

This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast flies and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq Jul 2008]

Gene Symbol:   HIRA
Gene Name:   HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
Aliases:   DGCR1, TUP1, TUPLE1
RefSeq:   NC_000022.10 NG_009231.1 NT_011519.10
Ensembl:   ENSG00000100084
Entrez:   7290
Chromosome Mapping:   22q11.2,22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.790000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download