PrimePCR™ SYBR® Green Assay: SEC31B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041629

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SEC31B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SEC31B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038907
Assay Design:   Intron-spanning
Chromosome Location:   10:102266137-102267258question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000370345 ENST00000451524 ENST00000370329 ENST00000535773 ENST00000310132 ENST00000585778

Gene Information

This gene encodes a protein of unknown function. The protein has moderate similarity to rat VAP1 protein which is an endosomal membrane-associated protein containing a putative Ca2+/calmodulin-dependent kinase II phosphorylation site. [provided by RefSeq Jul 2008]

Gene Symbol:   SEC31B
Gene Name:   SEC31 homolog B (S. cerevisiae)
Aliases:   DKFZp434M183, SEC31B-1, SEC31L2
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000075826
Entrez:   25956
Chromosome Mapping:   10q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.610000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download