PrimePCR™ SYBR® Green Assay: HMBS, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041561

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Assay Information

Technology:   qPCR
Assay Type   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038839
Assay Design:   intron_spanning
Chromosome Location:   11:118960936-118962848question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000328194 ENST00000530044 ENST00000278715 ENST00000537841 ENST00000542729 ENST00000546302 ENST00000442944 ENST00000544387 ENST00000543090 ENST00000539986 ENST00000535253 ENST00000392841 ENST00000372105 ENST00000293805 ENST00000570821 ENST00000571563 ENST00000575888 ENST00000571049 ENST00000574152 ENST00000571030 ENST00000572161 ENST00000574831 ENST00000574281 ENST00000575587

Gene Information

This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   HMBS
Gene Name:   hydroxymethylbilane synthase
Aliases:   PBG-D, PBGD, PORC, UPS
RefSeq:   NC_000011.9 NG_008093.1 NT_033899.8
Ensembl:   ENSG00000256269
Entrez:   3145
UniGene:   Hs.82609
Chromosome Mapping   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.780000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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