PrimePCR™ SYBR® Green Assay: ZNF775, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041507

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF775, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF775, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038785
Assay Design:   Intron-spanning
Chromosome Location:   7:150085510-150093664question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000328086 ENST00000444864 ENST00000550131 ENST00000520547 ENST00000522919 ENST00000380909 ENST00000303434 ENST00000222482 ENST00000493259 ENST00000445470 ENST00000300557 ENST00000257770 ENST00000369651 ENST00000478789 ENST00000329630 ENST00000490973 ENST00000258711 ENST00000432336 ENST00000550600 ENST00000388835 ENST00000388837 ENST00000375099 ENST00000507467 ENST00000564931

Gene Information

Description Not Available

Gene Symbol:   ZNF775
Gene Name:   zinc finger protein 775
Aliases:   MGC33584
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000196456
Entrez:   285971
Chromosome Mapping:   7q36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 35.900000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download