PrimePCR™ SYBR® Green Assay: CD70, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041410

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: CD70, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CD70, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038688
Assay Design:   Intron-spanning
Chromosome Location:   19:6586361-6590876question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000379803 ENST00000248054 ENST00000284486 ENST00000534308 ENST00000528897 ENST00000598234 ENST00000344649 ENST00000423145 ENST00000245903

Gene Information

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated but not on resting T and B lymphocytes. It induces proliferation of costimulated T cells enhances the generation of cytolytic T cells and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation cytotoxic function of natural killer cells and immunoglobulin sythesis. [provided by RefSeq Jul 2008]

Gene Symbol:   CD70
Gene Name:   CD70 molecule
Aliases:   CD27L, CD27LG, TNFSF7
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000125726
Entrez:   970
Chromosome Mapping:   19p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.160000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download