PrimePCR™ SYBR® Green Assay: AL359075.1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041405

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AL359075.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AL359075.1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038683
Assay Design:   Intron-spanning
Chromosome Location:   1:177915636-177917070question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000312917 ENST00000388715 ENST00000412770 ENST00000587283 ENST00000590348 ENST00000571995 ENST00000269347 ENST00000568433 ENST00000308284 ENST00000464631 ENST00000527976 ENST00000269197 ENST00000362091 ENST00000379999 ENST00000397269

Gene Information

SEC16B is a mammalian homolog of S. cerevisiae Sec16 that is required for organization of transitional endoplasmic reticulum (ER) sites and protein export (Bhattacharyya and Glick 2007 [PubMed 17192411]).[supplied by OMIM Jun 2009]

Gene Symbol:   AL359075.1
Gene Name:   Protein transport protein Sec16B
Aliases:   DKFZp686C2486, FLJ23871, FLJ25761, FLJ33652, FLJ36620, LZTR2, PGPR-p117, RGPR, SEC16S
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000120341
Entrez:   89866
Chromosome Mapping:   1q25.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.590000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download