PrimePCR™ SYBR® Green Assay: TM9SF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041380

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TM9SF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TM9SF1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038658
Assay Design:   Intron-spanning
Chromosome Location:   14:24662358-24663989question
Amplicon Length:   197
Splice Variants Targeted:   ENST00000216044 ENST00000420353 ENST00000456903 ENST00000328463 ENST00000415113 ENST00000263707 ENST00000409600 ENST00000321505 ENST00000389726 ENST00000300146 ENST00000555647 ENST00000556848 ENST00000354625 ENST00000225983 ENST00000336057 ENST00000393622 ENST00000261789 ENST00000528669 ENST00000524835 ENST00000396854 ENST00000528895 ENST00000530563 ENST00000530468 ENST00000525592 ENST00000528010 ENST00000556387 ENST00000530611

Gene Information

Description Not Available

Gene Symbol:   TM9SF1
Gene Name:   transmembrane 9 superfamily member 1
Aliases:   HMP70, MP70
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100926
Entrez:   10548
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.970000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download