PrimePCR™ SYBR® Green Assay: AGXT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041277

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AGXT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AGXT, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038555
Assay Design:   Intron-spanning
Chromosome Location:   2:241815379-241817529question
Amplicon Length:   200
Splice Variants Targeted:   ENST00000307503

Gene Information

This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes where it is involved in glyoxylate detoxification. Mutations in this gene some of which alter subcellular targetting have been associated with type I primary hyperoxaluria. [provided by RefSeq Jul 2008]

Gene Symbol:   AGXT
Gene Name:   alanine-glyoxylate aminotransferase
Aliases:   AGT, AGT1, AGXT1, PH1, SPAT, SPT, TLH6
RefSeq:   NC_000002.11 NG_008005.1 NT_005416.13
Ensembl:   ENSG00000172482
Entrez:   189
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.250000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download