PrimePCR™ SYBR® Green Assay: RUNX1T1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041276

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RUNX1T1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RUNX1T1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038554
Assay Design:   Intron-spanning
Chromosome Location:   8:93026907-93029478question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000523629 ENST00000396218 ENST00000360348 ENST00000422361 ENST00000520724 ENST00000518844 ENST00000521553 ENST00000518992 ENST00000521054 ENST00000519847 ENST00000517792 ENST00000522467 ENST00000517919 ENST00000521733 ENST00000520556 ENST00000521319 ENST00000520583 ENST00000523168 ENST00000436581 ENST00000265814

Gene Information

This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq Nov 2010]

Gene Symbol:   RUNX1T1
Gene Name:   runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
Aliases:   AML1T1, CBFA2T1, CDR, DKFZp564B213, ETO, FLJ33145, MGC2796, MTG8, ZMYND2
RefSeq:   NC_000008.10 NT_008046.16 NG_023272.1
Ensembl:   ENSG00000079102
Entrez:   862
Chromosome Mapping:   8q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.230000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download