PrimePCR™ SYBR® Green Assay: ARHGAP23, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041127

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARHGAP23, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARHGAP23, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038405
Assay Design:   Intron-spanning
Chromosome Location:   17:36614448-36619142question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000337299 ENST00000380518 ENST00000326724 ENST00000417379 ENST00000282397 ENST00000337929 ENST00000564089 ENST00000395754 ENST00000395755 ENST00000328333 ENST00000454817 ENST00000261712 ENST00000457654 ENST00000579451 ENST00000578379 ENST00000578892 ENST00000449662 ENST00000579788 ENST00000579687 ENST00000578313 ENST00000583366 ENST00000584084 ENST00000412356 ENST00000418105 ENST00000407721 ENST00000355527 ENST00000354216 ENST00000369892 ENST00000369908 ENST00000358711 ENST00000404910 ENST

Gene Information

The RHO (see ARHA; MIM 165390) family of small GTPases are involved in signal transduction through transmembrane receptors and they are inactive in the GDP-bound form and active in the GTP-bound form. GTPase-activating proteins such as ARHGAP23 inactivate RHO family proteins by stimulating their hydrolysis of GTP (Katoh and Katoh 2004 [PubMed 15254754]).[supplied by OMIM Mar 2008]

Gene Symbol:   ARHGAP23
Gene Name:   Rho GTPase activating protein 23
Aliases:   KIAA1501
RefSeq:   NC_000017.10 NT_010783.15 NW_003315948.1
Ensembl:   ENSG00000225485
Entrez:   57636
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.380000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download