PrimePCR™ SYBR® Green Assay: HAUS7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041084

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: HAUS7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HAUS7, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038362
Assay Design:   Intron-spanning
Chromosome Location:   X:152713333-152719938question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000330912 ENST00000338525 ENST00000334497 ENST00000370232 ENST00000370211 ENST00000435662 ENST00000370219 ENST00000370212 ENST00000421080 ENST00000303015 ENST00000520439 ENST00000597970 ENST00000596566 ENST00000595172 ENST00000595261 ENST00000597897 ENST00000598817 ENST00000599647 ENST00000595628 ENST00000600844

Gene Information

This gene encodes a protein identified by interaction with ubiquitin C-terminal hydrolase 37 which functions to edit polyubiquitin chains on ubiquitinated substrates. This protein is a subunit of the multisubunit augmin complex which regulates centrosome and mitotic spindle integrity and is necessary for the completion of cytokinesis. [provided by RefSeq Jul 2009]

Gene Symbol:   HAUS7
Gene Name:   HAUS augmin-like complex, subunit 7
Aliases:   HSXQ28ORF, UCHL5IP, UIP1
RefSeq:   NC_000023.10 NG_012688.1 NT_167198.1
Ensembl:   ENSG00000213397
Entrez:   55559
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.940000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download