PrimePCR™ SYBR® Green Assay: TRIM15, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041060

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TRIM15, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRIM15, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038338
Assay Design:   Intron-spanning
Chromosome Location:   6:30136266-30139619question
Amplicon Length:   194
Splice Variants Targeted:   ENST00000546888 ENST00000440260 ENST00000430090 ENST00000355703 ENST00000414873 ENST00000425255 ENST00000552062 ENST00000427091 ENST00000444157 ENST00000550694 ENST00000429524 ENST00000424597 ENST00000550702 ENST00000376694 ENST00000433744 ENST00000421984 ENST00000423220 ENST00000548732 ENST00000431059 ENST00000426765 ENST00000548653 ENST00000259930 ENST00000427952 ENST00000550969 ENST00000296137 ENST00000535325 ENST00000449058 ENST00000411850 ENST00000425375 ENST00000436462 ENST00000447733 ENST

Gene Information

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains a RING a B-box type 1 and a B-box type 2 and a coiled-coil region. The protein localizes to the cytoplasm. Alternatively spliced transcript variants have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   TRIM15
Gene Name:   tripartite motif containing 15
Aliases:   RNF93, ZNF178, ZNFB7
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204610
Entrez:   89870
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.090000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download