PrimePCR™ SYBR® Green Assay: RDBP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041048

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RDBP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RDBP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038326
Assay Design:   Intron-spanning
Chromosome Location:   6:31923023-31924739question
Amplicon Length:   185
Splice Variants Targeted:   ENST00000429857 ENST00000443464 ENST00000437732 ENST00000424967 ENST00000420837 ENST00000554752 ENST00000383343 ENST00000425810 ENST00000434518 ENST00000449057 ENST00000420039 ENST00000375429 ENST00000375425 ENST00000444811 ENST00000441998 ENST00000454913 ENST00000436289 ENST00000426722 ENST00000228862 ENST00000298573 ENST00000296733 ENST00000381375 ENST00000322374 ENST00000334206 ENST00000457397 ENST00000452147 ENST00000456263 ENST00000435435 ENST00000425721 ENST00000448628 ENST00000424762 ENST

Gene Information

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq Jul 2008]

Gene Symbol:   RDBP
Gene Name:   RD RNA binding protein
Aliases:   D6S45, NELF-E, RD, RDP
RefSeq:   NC_000006.11 NG_000013.3 NG_004658.2 NG_005163.2 NG_008191.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204356
Entrez:   7936
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.770000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download