PrimePCR™ SYBR® Green Assay: AKR1CL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040991

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AKR1CL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AKR1CL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038269
Assay Design:   Intron-spanning
Chromosome Location:   10:5204879-5227094question
Amplicon Length:   195
Splice Variants Targeted:   ENST00000445041 ENST00000555262 ENST00000449574 ENST00000308868 ENST00000430737 ENST00000422962 ENST00000547669 ENST00000549768 ENST00000278064 ENST00000357060 ENST00000428666 ENST00000458561 ENST00000414956 ENST00000547056 ENST00000549383 ENST00000376007 ENST00000376033 ENST00000454306 ENST00000432252 ENST00000550609 ENST00000550109 ENST00000439762 ENST00000416335 ENST00000550674 ENST00000548373 ENST00000401000 ENST00000369068 ENST00000596341 ENST00000435971 ENST00000428775 ENST00000551794 ENST

Gene Information

Description Not Available

Gene Symbol:   AKR1CL1
Gene Name:   aldo-keto reductase family 1, member C-like 1
Aliases:   FLJ16347
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000196326
Entrez:   340811
Chromosome Mapping:   10p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.620000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download