PrimePCR™ SYBR® Green Assay: NXF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040961

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: NXF2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NXF2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038239
Assay Design:   Intron-spanning
Chromosome Location:   X:101503513-101571929question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000547525 ENST00000332082 ENST00000549903 ENST00000261400 ENST00000361971 ENST00000538966 ENST00000268719 ENST00000368808 ENST00000341689 ENST00000567679 ENST00000371692 ENST00000371691 ENST00000372763 ENST00000372758 ENST00000372757 ENST00000395088 ENST00000330252 ENST00000340622 ENST00000458143 ENST00000372752 ENST00000372749 ENST00000372750 ENST00000457521 ENST00000412230 ENST00000354333 ENST00000350796 ENST00000303391 ENST00000272091 ENST00000336576 ENST00000243776 ENST00000355327 ENST

Gene Information

This gene is one of a family of nuclear RNA export factor genes. It encodes a protein that is involved in mRNA export is located in the nucleoplasm and is associated with the nuclear envelope. Alternative splicing seems to be a common mechanism in this gene family. Two variants have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   NXF2
Gene Name:   nuclear RNA export factor 2
Aliases:   CT39, FLJ20416, NXF2B, TAPL-2
RefSeq:   NC_000023.10 NT_011651.17
Ensembl:   ENSG00000185554
Entrez:   56001
Chromosome Mapping:   Xq22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.870000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download