PrimePCR™ SYBR® Green Assay: FUNDC2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040850

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FUNDC2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FUNDC2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038127
Assay Design:   Intron-spanning
Chromosome Location:   X:154279945-154282949question
Amplicon Length:   182
Splice Variants Targeted:   ENST00000585421 ENST00000536189 ENST00000262768 ENST00000586057 ENST00000374469 ENST00000401783 ENST00000302728 ENST00000381176 ENST00000369498 ENST00000353234 ENST00000375018 ENST00000540940 ENST00000373511 ENST00000373498 ENST00000350766 ENST00000373503 ENST00000265960 ENST00000394063 ENST00000373497 ENST00000257430 ENST00000508376 ENST00000457016 ENST00000598456

Gene Information

Description Not Available

Gene Symbol:   FUNDC2
Gene Name:   FUN14 domain containing 2
Aliases:   DC44, FLJ33773, HCBP6, HCC3, MGC131676, MGC2495, PD03104
RefSeq:   NC_000023.10 NG_005114.1 NG_011403.1 NT_167198.1
Ensembl:   ENSG00000165775
Entrez:   65991
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.140000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download