PrimePCR™ SYBR® Green Assay: PFDN5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040730

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PFDN5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PFDN5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038007
Assay Design:   Intron-spanning
Chromosome Location:   12:53691875-53693091question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000549759 ENST00000334478 ENST00000550846 ENST00000351500 ENST00000551018

Gene Information

This gene encodes a member of the prefoldin alpha subunit family. The encoded protein is one of six subunits of prefoldin a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides thereby allowing them to fold correctly. The complex consisting of two alpha and four beta subunits forms a double beta barrel assembly with six protruding coiled-coils. The encoded protein may also repress the transcriptional activity of the proto-oncogene c-Myc. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   PFDN5
Gene Name:   prefoldin subunit 5
Aliases:   MGC5329, MGC71907, MM-1, MM1, PFD5
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000123349
Entrez:   5204
Chromosome Mapping:   12q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.280000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download