PrimePCR™ SYBR® Green Assay: GPR89A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040715

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: GPR89A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GPR89A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037992
Assay Design:   Intron-spanning
Chromosome Location:   1:145804300-145811425question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000313835 ENST00000534502 ENST00000462900 ENST00000454423 ENST00000314163 ENST00000582624 ENST00000582339 ENST00000583232 ENST00000577475 ENST00000580001

Gene Information

GPR89A is a nearly identical copy of the GPR89B gene (MIM 612806).[supplied by OMIM Jun 2009]

Gene Symbol:   GPR89A
Gene Name:   G protein-coupled receptor 89A
Aliases:   FLJ10492, FLJ11696, FLJ36909, FLJ79270, GPR89B, MGC688, MGC87324, UNQ192
RefSeq:   NC_000001.10 NT_167185.1
Ensembl:   ENSG00000117262
Entrez:   653519
Chromosome Mapping:   1q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.550000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download