PrimePCR™ SYBR® Green Assay: TRIOBP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040677

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TRIOBP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRIOBP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037954
Assay Design:   Intron-spanning
Chromosome Location:   22:38147790-38150964question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000406386 ENST00000407319 ENST00000403663 ENST00000418339 ENST00000452519 ENST00000417857

Gene Information

This gene encodes a protein with an N-terminal pleckstrin homology domain and a C-terminal coiled-coil region. The protein interacts with trio which is involved with neural tissue development and controlling actin cytoskeleton organization cell motility and cell growth. The protein also associates with F-actin and stabilizes F-actin structures. Mutations in this gene have been associated with a form of autosomal recessive nonsyndromic deafness. Multiple alternatively spliced transcript variants that would encode different isoforms have been found for this gene however some transcripts may be subject to nonsense-mediated decay (NMD). [provided by RefSeq Nov 2008]

Gene Symbol:   TRIOBP
Gene Name:   TRIO and F-actin binding protein
Aliases:   DFNB28, FLJ39315, KIAA1662, TARA, dJ37E16.4
RefSeq:   NC_000022.10 NG_012857.1 NT_011520.12
Ensembl:   ENSG00000100106
Entrez:   11078
Chromosome Mapping:   22q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.380000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download