PrimePCR™ SYBR® Green Assay: FUT3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040619

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FUT3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FUT3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037896
Assay Design:   Intron-spanning
Chromosome Location:   19:5844738-5846497question
Amplicon Length:   156
Splice Variants Targeted:   ENST00000589918 ENST00000303225 ENST00000589620 ENST00000589714 ENST00000587048 ENST00000585715

Gene Information

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis tissue differentiation tumor metastasis inflammation and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(13)-fucosyltransferase and alpha(14)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   FUT3
Gene Name:   fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
Aliases:   CD174, FT3B, FucT-III, LE, Les, MGC131739
RefSeq:   NC_000019.9 NG_007482.1 NG_007505.1 NT_011255.14
Ensembl:   ENSG00000171124
Entrez:   2525
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.440000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download