PrimePCR™ SYBR® Green Assay: ZC3H14, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040608

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZC3H14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZC3H14, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037885
Assay Design:   Intron-spanning
Chromosome Location:   14:89076087-89077263question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000251038 ENST00000302216 ENST00000556945 ENST00000557607 ENST00000555755 ENST00000393514 ENST00000336693 ENST00000556000 ENST00000318308 ENST00000555900 ENST00000406216 ENST00000555792 ENST00000359301

Gene Information

ZC3H14 belongs to a family of poly(A)-binding proteins that influence gene expression by regulating mRNA stability nuclear export and translation (Kelly et al. 2007 [PubMed 17630287]).[supplied by OMIM Mar 2010]

Gene Symbol:   ZC3H14
Gene Name:   zinc finger CCCH-type containing 14
Aliases:   FLJ11806, MGC26892, MSUT-2, NY-REN-37, SUT2, UKp68
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100722
Entrez:   79882
Chromosome Mapping:   14q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.780000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download