PrimePCR™ SYBR® Green Assay: GIT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040605

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GIT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GIT2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037882
Assay Design:   Intron-spanning
Chromosome Location:   12:110370971-110376281question
Amplicon Length:   156
Splice Variants Targeted:   ENST00000355312 ENST00000457474 ENST00000361006 ENST00000553118 ENST00000551209 ENST00000552978 ENST00000550186 ENST00000546613 ENST00000548000 ENST00000360185 ENST00000354574 ENST00000338373 ENST00000343646 ENST00000356259

Gene Information

This gene encodes a member of the GIT protein family which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes focal adhesions and the cell periphery and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq Sep 2008]

Gene Symbol:   GIT2
Gene Name:   G protein-coupled receptor kinase interacting ArfGAP 2
Aliases:   CAT-2, CAT2, DKFZp686G01261, KIAA0148, MGC760
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000139436
Entrez:   9815
Chromosome Mapping:   12q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.290000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download